Journal: Antioxidants

Article Title: Bioenergetic Signatures of DLD Deficiency: Dissecting PDHc- and α-KGDHc-Linked Defects

doi: 10.3390/antiox15010019

Figure Lengend Snippet: Mitochondrial respiration is impaired in fibroblasts derived from patients with DLD deficiency. Mitochondrial oxygen consumption was assessed in controls (Ctrl1 and Ctrl2) and patient (Pt1–Pt6) fibroblasts using high-resolution respirometry (Oroboros O2k). ( A ) Routine respiration; ( B ) maximal respiration calculated as the difference between FCCP-stimulated and α-chaconine–permeabilized rates; ( C ) complex I-linked respiration (NADH-linked respiration, N-pathway), calculated as the difference between ADP and α-chaconine; ( D ) complex II-linked respiration (NS-pathway) calculated as the difference between respiration after rotenone and α-chaconine addition; ( E ) effect of complex I inhibition, calculated as the difference between FCCP-stimulated and rotenone-inhibited respiration; and ( F ) complex I/complex II respiration ratio (complex I-linked activity divided by complex II-linked activity). Each open circle represents an independent experimental run (N = 4–8 repeats per sample). All data were normalized to cell number. Statistical analysis was performed using the Mann–Whitney U test. * p < 0.05 vs. Ctrl1; numerical p values (0.05 < p < 0.1) are indicated on the plots. Abbreviations: Ctrl, control; Pt, patient.

Article Snippet: Dermal fibroblast primary cell lines from six genetically confirmed patients with DLD deficiency were obtained from the Pediatric Metabolic Disease Unit, Sheba Medical Center (IRB# SMC-21-8644, Figure 1, Table 1, and ), as well as two control cell lines: a control human dermal fibroblast cell line was purchased from ATCC (PCS-201-012; Ctrl 1, Manassas, VA, USA), and a primary cell line from a 39-year-old healthy male (Ctrl 2).

Techniques: Derivative Assay, Inhibition, Activity Assay, MANN-WHITNEY, Control

Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Viability of human dermal fibroblasts (HDFa), breast adenocarcinoma cells (MCF-7), and colorectal adenocarcinoma cells (Caco-2) after 48 h exposure to arenin. Data are expressed as the percentage of MTS-formazan absorbance (490 nm) relative to untreated controls (mean ± SD, n = 3). Statistical analysis was performed with one-way ANOVA followed by Tukey’s multiple-comparison test; Different letters abcde indicate statistical differences analyzed using the Tukey test ( p < 0.05)

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Comparison

Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Effect of arenin on HDFa cell migration under high glucose (HG) and serum-deprived (FBS–) conditions. Scratch wound healing assays were performed in monolayers treated with arenin (31.25–1000 µg mL −1 ), Centella asiatica extract (CA, 10 µg mL −1 ), or left untreated (C–). A , C Time course of wound retraction (%) under FBS– and HG conditions at 24, 48, and 72 h. B , D Wound closure (%) after 72 h in FBS– and HG conditions. E Phase contrast micrographs at 0 and 72 h under FBS– (upper) and HG (lower) conditions show C–, CA, and arenin treatments. Dashed lines separate controls from arenin. Complete closure is observed in arenin- and CA-treated cultures, while sizeable gaps persist in C–. Bars/data points = mean ± SD ( n = 3). One-way ANOVA with Tukey’s post hoc test; groups sharing the same lowercase letter are not significantly different ( p > 0.05). Scale bars = 200 µm

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Migration